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Leica SP8 Confocal LSM

Formerly known as the Advanced Microscopy Platform

Description

The MultiPark Microscopy Platform currently has a Leica SP8 laser scanning confocal microscope for imaging live and fixed tissue and cells. The system is also equipped with a live imaging chamber, which can be used with additional training. The system is located in BMC A1054 and has been up and running since late 2019.

Using the system

Training

All users must first receive training in order to gain access to the system. This includes potential users who have previously used a similar system, such as the system in A11. Use of the system without training is prohibited and may result in loss of access.

Training takes approximately 4 hours and can be scheduled by contacting Megg (megg [dot] garcia [at] med [dot] lu [dot] se (megg[dot]garcia-ryde[at]med[dot]lu[dot]se)).

The cost for training is a base 1000 SEK plus the cost of 4 hours of usage (200 SEK/hour), which totals to 1800 SEK.

Booking

Booking the system via the Outlook calendar and logging usage in the logbook are required.

Booking is done ahead of time via an Outlook calendar specific for this system. The address for the booking calendar is MED_A10-Confocal-mp [at] resource [dot] lu [dot] se (MED_A10-Confocal-mp[at]resource[dot]lu[dot]se). Please note that only users who have undergone training are allowed to book the system. Booking priority is first come, first served with the exception being system maintenance or repairs, which take priority over all user bookings.

Once at the system, users must fill in an entry in the log sheet located near the system with the required information.


Below is a table highlighting key points for the system:

Objectives
  • HC PL Fluotar 5x/Air NA 0.15
  • HC PL APO 20x CS2/Air NA 0.75 
  • HC PL APO 40x CS2/Oil NA 1.30
  • HC PL 63x CS2/Oil NA 1.40
Laser lines405, 488, 552 and 638 nm
Detectors1 x Photomultiplier tube (PMT) and 1 x Leica hybrid detector (HyD)
Live cell imagingOkolab stage-top chamber system (additional training required)
User fee200 SEK/hour
BookingVia Outlook calendar
Training fee1000 SEK + 4 hours of usage (1800 SEK total)
Contact for training/accessMegg Garcia-Ryde 
megg [dot] garcia-ryde [at] med [dot] lu [dot] se
Primary responsibleGunnar Gouras
gunnar [dot] gouras [at] med [dot] lu [dot] se

 

Partial view of a microscopy system with an inverted microscope to the left, microscopy control boxes in the middle, and a computer screen, keyboard, and control panel to the right.
The Leica SP8 laser scanning confocal microscope system located in BMC A1054.

 

Contact for training/access

Girl smiling with head tilted slightly

Megg Garcia-Ryde
Research engineer
Email: megg [dot] garcia-ryde [at] med [dot] lu [dot] se
Office: BMC B1128b

Responsible PI

Gunnar Gouras
Professor
Email: gunnar [dot] gouras [at] med [dot] lu [dot] se
Office: BMC B1123c