Operetta CLS

High content screening (HCS) / Analysis (HCA) enables automated acquisition, visualization and quantification of a large number of images and can capture and measure dynamic processes, such as migration and/or changes in morphology, expression pattern and/or intensity.

This system features

• Automated fluorescence and brightfield microscopy
• Widefield and confocal (spinning disc) mode
• Environmental control (temperature and CO₂)
• Extraction of quantitative data from intensity and area measurements
• High throughput format

With this system, you have the capability to image different types of samples and capture different targets, such as

• Live and fixed cells
• Subcellular targets
• Small organisms

For more details about the system, you can click here to open the Operetta CLS webpage on revvity's website.

Booking

All new users must first receive training in order to gain access to the system. This can be arranged by contacting Anna Hammarberg via email (anna [dot] hammarberg [at] med [dot] lu [dot] se). 

Once training is completed, users can book the system using the Infinity X online system, which can be accessed via this link: https://infinity.ideaelan.com/lund/auth/login. 

Users log in using their Lucat-ID and password and must then have access to both the facility "MultiPark Infrastructures" and the instruments "Operetta CLS" and "Harmony Office". A quick guide to requesting instrument access can be found here.

Booking follows a first come, first served policy. In general, booking 10 days in advance should give you your desired time slot. However, do not hesitate to book the system well in advance nor ask for last-minute service if needed.

User fees

For MultiPark members:
300 SEK/hour of scan time
300 SEK/hour for introduction and protocol setup

For users external to MultiPark:
300 SEK/hour of scan time
300 SEK/hour for introduction and protocol setup

Introduction to the Operetta CLS instrument and Harmony software takes approximately 3-4 hours. Contact Anna Hammarberg for training.

Services available

Assistance with

• Setting up image acquisition
• Setting up analysis protocols
• Assay validation
• Troubleshooting
• Developing new protocols and techniques
• Running experiments and analyzing data together with the user

Training, including

• Training for image acquisition and protocol development and optimization
• Production of tutorials and literature
• Organization of theoretical and practical workshops

Planning - contact Anna Hammarberg and book a time for consultation and planning

Execution - please note the following:

• All sample preparation should be done by the user group
• Consumables for sample preparation are not included in the user fee
• Assistance with data acquisition and analysis may be provided in small project and pilot experiments. For repeated experiments, the user is gradually trained to become independent in image acquisition, protocol optimization, data evaluation and analysis. Assistance will be available for instruction and trouble shooting.

Publishing guidelines

As with all MultiPark infrastructures, platforms and instruments, when publishing any data that used this Operetta CLS system, the user must mention MultiPark in the acknowledgement section.