Quanterix SR-X Ultra-Sensitive Biomarker Detection System
Simoa is particularly useful for measurements of biomarkers at ultra-low levels. The approach is based on the counting of thousands of single immunocomplexes in femtoliter-sized reaction chambers which are termed Simoa - Single Molecule Arrays. Simoa uses the same reagents as conventional ELISA, but antibody capture agents are attached to the surface of paramagnetic beads that are used to concentrate a dilute solution of target molecules. The beads are loaded to discs containing arrays of femtoliter-sized wells that hold only one bead per well. In wells containing a bead with a single target molecule, the formation of an immunocomplex will result in enzymatic cleavage of a substrate, which will be converted to a fluorescent product imaged by the SR-X reader.
The Simoa allows:
- Single molecule array technique for high sensitivity and broad dynamic range
- Combined digital and analog immunoassay dynamic range is >4 logs
- A range of sample types include serum, plasma, cerebrospinal fluid, urine, cell extracts
- Fexibility to design assays to detect both protein and nucleic acid biomarkers
- Detection of nucleic acid levels, including miRNA, without PCR
- Single and multiplex assays
- Multiplexing up to six analytes
- Semi-automated assay preparation using standardized benchtop devices
- Simple 2-step or 3-step workflow with minimal user intervention
- Low volume requirements
- Wide menu of available kits
- Full line of components for user-developed assays
A menu of Simoa assay kits (single plex and multiplex) will be available to measure critical biomarkers. It is also possible to request a custom designed assay.
Examples of multiplexing Simoa Assay Kits for SR-X:
- Neurology 3-Plex A (Tau, Aß42, Aß40)
- Neurology 4-Plex A (Tau, NF-Light, GFAP, UCH-L1)
- Cytokine 3-Plex A NP (IL-10, IL-6, TNFα)
To learn more, visit the official Quanterix SR-X website
The Simoa is available for everyone to use.
E-mail: pilar [dot] lorenzo [at] med [dot] lu [dot] se
Location: B1113 (lab)