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Next Generation Sequencing (NGS)

The MultiPark next generation sequencing (NGS) facility is located at the BMC in Lund. The core offers state-of-the art next generation sequencing technology for experimental and preclinical studies with particular expertise in transcriptome analysis.  It is a core facility providing you with a full service – from RNA isolation to initial bioinformatics analysis.

The facility is open to all MultiPark researchers, Lund University affiliates and external users. The services provided include:

  • RNA isolation
  • Initial QC of RNA/DNA (BioAnalyzer +Qubit)
  • Library preparation
  • QC of sequencing libraries (BioAnalyzer +Qubit)
  • Next generation sequencing (the core has a Illumina NextSeq500 instrument)
  • Data storage
  • Initial bioinformatics analysis

In addition to RNA-seq, other NGS-based techniques such as various forms of DNA-analysis and ChIP-seq are available upon request.

The core has expertise in handling many different types of tissues and cells, including the analysis of samples with very small amount of RNA.


Charges
Use of the core facility is free of charge and users only pay for reagents. Approximate costs for one single RNA-seq sample typicalle range from 1000 SEK – 3000 SEK per sample but are dependent on the design of the project.


Consultation and contact
Before planning your experiments we advice you to consult us:

Jenny G Johansson
E-mail: Jenny_G [dot] Johansson [at] med [dot] lu [dot] se
Phone: +46 46 222 05 54

Yogita Sharma
E-mail: Yogita [dot] Sharma [at] med [dot] lu [dot] se
Phone: 046-222 34 18

Contact

Jenny G Johansson
Research engineer

E-mail: Jenny_G [dot] Johansson [at] med [dot] lu [dot] se
Phone: +46 46 222 05 54


Yogita Sharma
Bioinformatician

E-mail: Yogita [dot] Sharma [at] med [dot] lu [dot] se
Phone: +46 46 222 34 18