Two-photon microscopy is the method of choice for imaging living cells and tissues (intravital microscopy). It allows imaging with very high temporal and spatial resolution in thick biological material. This MultiPark platform consists of two microscopy stations that serve different needs. The ‘in vivo’ setup can be used to image the processes in the living mouse, i.e. superficial layers of the cerebral cortex. The ‘ex vivo’ system is designed to image thick live preparations, such as brain regions that are not accessible to ‘in vivo’ microscopy. In this setup, two-photon imaging can also be combined with patch-clamp electrophysiological recordings. Although primarily acquired to perform studies in neuroscience, the system is available to all interested researchers at and outside LU.