Flow cytometry enables measurement of fluorescence intensity of particles (cells) in a liquid stream. Analysis of live and fixed cells may be based on size, granularity/complexity, fluorescent proteins, cell surface antigen expression, intracellular antigen expression, DNA content. Flow cytometry is ideal for multiplexing targets using multiple stainings and dyes.
We have two instruments for flow cytometry analysis and sorting:
Cell sorter: BD FACS Aria III
3 LASER instrument: 488 nm, 561 nm, 633 nm; 10 fluorescence channels. The FACS Aria may be used for analysis or for physically separating out cell populations based on their fluorescence labelling.
• Purity sort
• Enrichment of rare populations
• Single cell sort
Analyzer: BD Accuri C6
Easy to use 2 LASER instrument: 488 nm, 640 nm; 4 fluorescence channels
• Particle count and volumetric measurement
If you are interested in Flow Cytometry/FACS, or just want to know more about these instruments, please contact anna [dot] hammarberg [at] med [dot] lu [dot] se (Anna Hammarberg), MultiPark - Cellomics and Flow Cytometry Core Facility.
The FACS facility is available for everyone to use.
E-mail: Anna [dot] Hammarberg [at] med [dot] lu [dot] se
Tel: +46 46 222 0642
Location: B1135a (lab) or B1131a (office)