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Platforms

F09 MultiPark Platform

The MultiPark set-up (MP set-up) is based on a Molecular Devices Axopatch 700B amplifier with the Digidata 1440 digitizer. This set-up is more suitable for brain slice preparations, allowing for up to 2-channel recordings in combination with electrical stimulation and/or confocal/2-photon imaging, if required. The rig is integrated with a Zeiss LSM 710 NLO microscope, offering functionalities for both confocal and 2-photon laser imaging. For more information about this imaging setup, supported by MultiPark, please visit  the two-photon microscope webpage

Major applications offered to the investigators on MultiPark platform include:

  • Patch clamp recording ex vivo (fresh tissue preparations) and, to some extent in vitro(cell cultures), to study intrinsic electrical properties of single cells, such as resting membrane potential, membrane resistance and capacitance, action potential parameters, presence and function of various channels and receptors as well as synaptic connectivity, and to identify, characterize and quantify synaptic transmission in neurons. 
  • Extracellular field potential recordings, spontaneous or evoked, to study general network activity, or synaptic plasticity ex vivo in brain tissue preparations (predominantly in hippocampus – LTP, LTD)
  • Dual recordings, concurrent double field, double single-cell or concurrent single-cell and local field recordings, to correlate network activity between two locations or in surrounding areas of the patched neuron. 
    F09 electrophysiology rig
    F09 electrophysiology rig

B10 StemTherapy Platform

The Stem Therapy set-up consists of a HEKA EPC10 double-patch clamp amplifier, an Olympus BX51WI microscope with multi-channel fluorescence capability and differential interference contrast for live imaging and optical manipulation of cells (e.g. optogenetics and calcium imaging), and a Hamamatsu C11440-10C camera. This set-up is also equipped with a Multielectrode Array (MEA) system and there is a possibility to perform micromanipulator assisted collection of single cells for qPCR (Patch-Seq).  Located on B10, inside the cell culture laboratory, this set-up is ideal for, but not restricted to, investigation of in vitro generated and expanded cell lines and cultures.

Major applications offered to the investigators on Stem Therapy platform include:

  • Patch clamp recording in vitro (cell cultures) and, to some extent, ex vivo (tissue preparations) to study intrinsic electrical properties of single cells, such as resting membrane potential, membrane resistance, action potential parameters, presence and function of various channels and receptors as well as synaptic connectivity, and to identify, characterize and quantify synaptic transmission. Our main focus is neural lineage (e.g. neurons and astrocytes), but other cell types can also be recorded (e.g. cardiomyocytes, β cells)
  • Extracellular field potential recordings, spontaneous or evoked, to study general network activity, or synaptic plasticity ex vivo in brain tissue preparations (predominantly in hippocampus – LTP, LTD)
  • Optogenetic manipulation of cell function to study, e.g. synaptic profile of isolated populations of the cells in cell cultures and tissue preparations 
  • Optical imaging of live cells in cultures and tissue preparations to monitor intracellular Ca2+ fluctuations and to identify population of interest
  • Multielectrode Array (MEA) recordings of extracellular field potentials from cell cultures and tissue preparations

*Please note: The facility is located inside the cell culture lab and everyone wishing to use the set-up has to get access to the lab. Researchers have to contact the responsible people and get the training and access to the lab on their own.

B10 elecrtrophysiology rig
B10 elecrtrophysiology rig
Apostolos Mikroulis. Photo.

Apostolos Mikroulis, PhD
Research engineer at F09

apostolos [dot] mikroulis [at] med [dot] lu [dot] se
Phone +46 (0)4 62 22 36 19

Natalia Avaliani. Photo.

Natalia Avaliani, PhD
Facility Manager at B10

natalia [dot] avaliani [at] med [dot] lu [dot] se
Phone: +46 46 2220515
Mobile: +45 31212235